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    Examining the Synergism of Cerium Oxide Nanoparticle – Andrographolide Conjugate and Studying its Potential Role Towards Antioxidative Therapeutics

    • Sneha Kumari
    • Shivam Pandey
    • Leela Manohar Aeshala
    • Anuj Kumar
    • Sushant Singh


    Abstract

    Increased oxidative stress in metabolic disorders has revealed promising alternatives for antioxidative therapeutics. Herein, we present synthesis and evaluations of Cerium oxide nanoparticles (CNPs) conjugated with Andrographolide (Ad-CNP). Characterization revealed a uniform particle size of 106 nm for bare CNP and 120 nm for Ad-CNP conjugates. UV-visible spectroscopy showed an absorbance peak at 230nm, indicating a high Ce³⁺/Ce⁴⁺ oxidation state. X-ray diffraction confirmed a pure cubic fluorite structure with a polycrystalline nature with peaks at 111, 200, 220, and 311. In terms of reactive oxygen species scavenging, bare CNPs demonstrated Catalase mimetic activity of 41.5%, whereas the Ad-CNP conjugates showed 47% scavenging activity. The Superoxide Dismutase mimetic activity was significantly increased due to synergy up to 77% of Ad-CNP whereas bare CNPs showed 57% activity.  The CNPs exhibited notable antibacterial efficacy, diminishing microbial proliferation by 69% for bare CNPs and 82% for Ad-CNP. Biocompatibility testing with human skin keratinocytes validated the safety of the nanoparticles, and anti-inflammatory assays indicated decreased expression of pro-inflammatory cytokines IL-6 and TNF-α. The synergistic combination of CNPs with Andrographolide demonstrated significant potential as a potent antioxidant, antibacterial, and anti-inflammatory agent, making it a prospective candidate for antioxidative therapeutic research aimed at diminishing oxidative stress.

    Keywords: Cerium oxide Nanoparticles Andrographolide Catalase Superoxide Dismutase Biocompatibility
    Section

    References

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    Examining the Synergism of Cerium Oxide Nanoparticle – Andrographolide Conjugate and Studying its Potential Role Towards Antioxidative Therapeutics. (2024). Nanofabrication, 9. https://doi.org/10.37819/nanofab.9.2031
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    Examining the Synergism of Cerium Oxide Nanoparticle – Andrographolide Conjugate and Studying its Potential Role Towards Antioxidative Therapeutics. (2024). Nanofabrication, 9. https://doi.org/10.37819/nanofab.9.2031

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    Sneha Kumari
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    Shivam Pandey
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    Leela Manohar Aeshala
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    Anuj Kumar
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    Copyright (c) 2024 Sneha Kumari, Shivam Pandey, Leela Manohar Aeshala, Anuj Kumar, Sushant Singh

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