Skip to main content Skip to main navigation menu Skip to site footer
  • \
    Start Submission Become a Reviewer

    Fabrication and characterization of high molecular mass tmpe-based polyurethane wound dressing materials containing allantoin and gentamicin by electrospinning

    • Ayşe Başak Çakmen
    • Samir Abbas Ali Noma
    • Canbolat GÜRSES
    • Süleyman Köytepe
    • Burhan Ateş
    • İsmet Yılmaz


    Abstract

    In this study, biocompatible, antibacterial and high mechanical strength polyurethane-based wound dressing materials were prepared by using the electrospinning technique. In addition, allantoin and gentamicin which will contribute to wound healing, were incorporated into these fiber materials. Polyurethane structures containing trimethylolpropane ethoxylate (TMPE) with 2 different molecular weights were synthesized. TMPE-based polyurethanes/polycaprolactone (1:3) blends were also prepared by adding 1% gentamicin and 10% allantoin and they were knitted by the electrospinning method and turned into a wound dressing material. After this stage, chemical structure, morphological, thermal and mechanical properties, flexibility, antibacterial effect, in vitro biocompatibility, cell adhesion tests, allantoin release level, and biodegradability of the prepared wound dressing materials were performed. The prepared fiber materials exhibited antibacterial properties and 80% cell viability, approximately. In addition, the obtained wound dressing materials showed high mechanical strength and ideal gas permeability. For this reason, it offers an ideal alternative for closing wounds.

    Keywords: Biomaterials Wound dressing material Biocompatibility PU fibers Electrospinning
    Section

    References

    1. BHOYAR, S.D., MALHOTRA, K., & MADKE, B. (2023). Dressing Materials: A Comprehensive Review. J. Cutan. Aesthet. Surg., 16(2), 81-89. https://doi.org/10.4103/JCAS.JCAS_163_22
    2. BOATENG, J.S., MATTHEWS, K.H., STEVENS, H.N.E., & ECCLESTON, G.M. (2008). Wound Healing Dressings and Drug Delivery Systems: A Review. Indian J. Pharml. Sci., 97, 2892–2923. https://doi.org/10.1002/jps.21210
    3. CHATURVEDI, A., BAJPAI, A.K., BAJPAI, J., & SINGH, S.K. (2016). Evaluation of poly (vinyl alcohol) based cryogel-zinc oxide nanocomposites for possible applications as wound dressing materials. Mater. Sci. Eng., 65, 408-418. https://doi.org/10.1016/j.msec.2016.04.054
    4. CHEN, J.P., & CHIANG, Y. (2010). Bioactive electrospun silver nanoparticles-containing polyurethane nanofibers as wound dressings. J. Nanosci. Nanotechnol., 10, 7560-7564. https://doi.org/10.1166/jnn.2010.2829
    5. CHITRATTHA, S., & PHAECHAMUD, T. (2016). Porous poly(dl-lactic acid) matrix film with antimicrobial activities for wound dressing application. Mater. Sci. Eng., 58, 1122-1130. https://doi.org/10.1016/j.msec.2015.09.083
    6. DEITZEL, J.M., KLEINMEYER, J.D., HIRVONEN, J.K., & BECK TAN, N.C. (2001). Controlled deposition of electrospun poly(ethylene oxide) fibers. Polymer, 42, 8163-8170. https://doi.org/10.1016/S0032-3861(01)00336-6
    7. DEMARRE, L., VERHAEGHE, S., VAN HECKE, A., CLAYS, E., GRYPDONCK, M., & BEECKMAN, D. (2015). Factors predicting the development of pressure ulcers in an at-risk population who receive standardized preventive care: secondary analyses of a multicentre randomised controlled trial. J. Adv. Nurs., 71, 391–403. https://doi.org/10.1111/jan.12497
    8. DHIVYA, S., PADMA, V.V., & SANTHINI, E. (2015). Wound dressings - a review. Biomedicine, 5(4), 22. https://doi.org/10.7603/s40681-015-0022-9
    9. ESKANDARINIA, A., KEFAYAT, A., AGHEB, M., RAFIENIA, M., BAGHBADORANI, M.A., NAVID, S., EBRAHIMPOUR, K., KHODABAKHSHI, D., & GHAHREMANI, F. (2020). A novel bilayer wound dressing composed of a dense polyurethane/propolis membrane and a biodegradable polycaprolactone/gelatin nanofbrous scafold. Scientific Reports, 10, 3063. https://doi.org/10.1038/s41598-020-59931-2
    10. ESKANDARINIA, A., KEFAYAT, A., GHARAKHLOO, M., AGHEB, M., KHODABAKHSHI, D., KHORSHIDI, M., SHEIKHMORADI, V., RAFIENIA, M., & SALEHI, H. (2020). A propolis enriched polyurethane-hyaluronic acid nanofibrous wound dressing with remarkable antibacterial and wound healing activities. International Journal of Biological Macromolecules, 149, 467-476. https://doi.org/10.1016/j.ijbiomac.2020.01.255
    11. HARYŃSKA, A., KUCINSKA-LIPKA, J., SULOWSKA, A., GUBANSKA, I., KOSTRZEWA, M., & JANIK, H. (2019). Medical-grade PCL based polyurethane system for FDM 3D printing—characterization and fabrication. Materials, 12, 887. https://doi:10.3390/ma12060887
    12. HEYER, K., AUGUSTIN, M., PROTZ, K., HERBERGER, K., SPEHR, C., & RUSTENBACH, S. J. (2013). Effectiveness of advanced versus conventional wound dressings on healing of chronic wounds: systematic review and meta-analysis. Dermatology, 226, 172–184. https://doi.org/10.1159/000348331
    13. KIM, S., PARK, S.G., KANG, S.W., & LEE, K.J. (2016). Nanofiber‐based hydrocolloid from colloid electrospinning toward next generation wound dressing. Macromol. Mater. And Eng., 301, 818-826. https://doi.org/10.1002/mame.201600002
    14. KİMEL, K., GODLEWSKA, S., GLEŃSK, M., GOBİS, K., OŚKO, J., GREMBECKA, M., & KRAUZE-BARANOWSKA, M. (2023). LC-MS/MS evaluation of pyrrolizidine alkaloids profile in relation to safety of comfrey roots and leaves from Polish sources. Molecules, 28, 6171. https://doi.org/10.3390/molecules28166171
    15. LEE, S.J., HEO, D.N., MOON, J.H., PARK, H.N., KO, W.K., BAE, M.S., LEE, J.B., PARK, S.W., KIM, E.C., LEE, C.H., JUNG, B.Y., & KWON, I.K. (2014). Chitosan/polyurethane blended fiber sheets containing silver sulfadiazine for use as an antimicrobial wound dressing. J. Nanosci. Nannotechnol., 14, 7488-7494. https://doi.org/ 10.1166/jnn.2014.9581
    16. LIONELLI, G.T., & LAWRENCE, W.T. (2003). Wound dressings. Surg. Clin. North Am., 83, 617-638.
    17. LIU, M., DUAN, X.P., LI, Y.M., YANG, D.P., & LONG, Y.Z. (2017). Electrospun nanofibers for wound healing. Materials Sci. and Eng. C., 76, 1413-1423. https://doi.org/10.1016/j.msec.2017.03.034.
    18. MEHTEROĞLU, E., ÇAKMEN, A., AKSOY, B., BALCIOĞLU, S., KÖYTEPE, S., ATEŞ, B., & YILMAZ, İ. (2020). Preparation of hybrid PU/PCL fibers from steviol glycosides via electrospinning as a potential wound dressing materials. Journal of Applied Polymer Science, 49217. https://doi.org/10.1002/app.49217
    19. MIGUEL, S.P., RIBEIRO, M.P., BRANCAL, H., COUTINHO, P., & CORREIA, I.J. (2014). Thermoresponsive chitosan–agarose hydrogel for skin regeneration. Carbohydr. Polym., 111, 366-373. https://doi.org/10.1016/j.carbpol.2014.04.093
    20. MOGOŞANU, G.D., & GRUMEZESCU, A.M. (2014). Natural and synthetic polymers for wounds and burns dressing. Int. J. Pharm., 463, 127-136. https://doi.org/10.1016/j.ijpharm.2013.12.015
    21. NASTİĆ, N., BORRÁS-LİNARES, I., LOZANO-SÁNCHEZ, J., ŠVARC-GAJİĆ, J., & SEGURA-CARRETERO, A. (2020). Comparative assessment of phytochemical profiles of comfrey (Symphytum officinale L.) root extracts obtained by different extraction techniques. Molecules, 25(4), 837. https://doi.org/10.3390/molecules2504083
    22. NGUYEN, H.M., LE, T.T.N., NGUYEN, A.T., LE, H.N.T., & PHAM, T.T. (2023). Biomedical materials for wound dressing: recent advances and applications. RSC Adv., 13, 5509-5528. https://doi.org/10.1039/D2RA07673J
    23. PİERCHALA, M.K., MAKAREMİ, M., TAN, H.L., PUSHPAMALAR, J., MUNİYANDY, S., SOLOUK, A., LEE, S.M., & PASBAKHSH, P. (2018). Nanotubes in nanofibers: Antibacterial multilayered polylactic acid/halloysite/gentamicin membranes for bone regeneration application. App. Clay Sci., 160, 95-105. https://doi.org/10.1016/j.clay.2017.12.016
    24. PYUN, D.G., CHOI, H.J., YOON, H.S., THAMBI, T., & LEE, D.S. (2015). Polyurethane foam containing rhEGF as a dressing material for healing diabetic wounds: synthesis, characterization, in vitro and in vivo studies. Colloids Surf., 135, 699-706. https://doi.org/10.1016/j.colsurfb.2015.08.029
    25. SAHRARO, M., YEGANEH, H., & SORAYYA, M. (2016). Guanidine hydrochloride embedded polyurethanes as antimicrobial and absorptive wound dressing membranes with promising cytocompatibility. Mater. Sci. Eng., 59, 1025-1037. https://doi.org/10.1016/j.msec.2015.11.038
    26. SİNGH, B., SHARMA, S., & DHİMAN, A. (2013). Design of antibiotic containing hydrogel wound dressings: Biomedical properties and histological study of wound healing. Int J. Pharm., 457, 82-91. https://doi.org/10.1016/j.ijpharm.2013.09.028
    27. STAİGER, C. (2013). Comfrey root: From tradition to modern clinical trials. Wien. Med. Wochenschr., 163, 58–64. https://doi.org/10.1007/s10354-012-0162-4
    28. XU, R., LUO, G., XIA, H., HE, W., ZHAO, J., LIU, B., TAN, J., ZHOU, J., LIU, D., WANG, Y., YAO, Z., ZHAN, R., YANG, S., & WU, J. (2015). Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction. Biomaterials, 40, 1-11. https://doi.org/10.1016/j.biomaterials.2014.10.077
    29. XU, R., XIA, H., HE, W., LI, Z., ZHAO, B., LIU, B., WANG, Y., LEI, Q., KONG, Y., BAI, Y., YAO, Z., YAN, R., LI, H., ZHAN, R., YANG, S., LUO, G., & WU, J. (2016). Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement. Sci. Rep., 6, 24596. https://doi.org/10.1038/srep24596
    30. XU, W.T., MA, C.F., MA, J.L., GAN, T.S., & ZHANG, G.Z. (2014). Marine biofouling resistance of polyurethane with biodegradation and hydrolyzation. ACS Appl. Mater. Interfaces., 6, 4017-4024. https://doi.org/10.1021/am4054578
    31. YAO, C.H., LEE, C.Y., HUANG, C.H., CHEN, Y.S., & CHEN, K.Y. (2017). Novel bilayer wound dressing based on electrospun gelatin/keratin nanofibrous mats for skin wound repair. Mater. Sci. Eng. C Mater. Biol. Appl., 79, 533-540. https://doi.org/10.1016/j.msec.2017.05.076
    32. YAN, L., SI, S., CHEN, Y, YUAN T., FAN H., YAO Y., ZHANK Q. (2011) Electrospun in-situ hybrid polyurethane/nano-TiO2 as wound dressings. Fibers Polym. 12, 207–213. https://doi.org/10.1007/s12221-011-0207-0.
    33. YUDANOVA, T.N., & RESHETOV, I.V. (2006). Modern wound dressings: Manufacturing and properties. Pharm. Chem. J., 40, 85-92.
    34. ZAHEDI, P., REZAEIAN, I., RANAEI-SIADAT, S.O., JAFARI, S.H., & SUPAPHOL, P. (2009). A review on wound dressings with an emphasis on electrospun nanofibrous polymeric bandages. Polym. Adv. Technol., 21, 77-95. https://doi.org/10.1002/pat.1625

    How to Cite

    Çakmen, A. B., Noma, S. A. A., GÜRSES, C., Köytepe, S., Ateş, B., & Yılmaz, İsmet. (2023). Fabrication and characterization of high molecular mass tmpe-based polyurethane wound dressing materials containing allantoin and gentamicin by electrospinning. Nanofabrication, 8. https://doi.org/10.37819/nanofab.8.1787
    ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
    Download Citation
    Endnote/Zotero/Mendeley (RIS) BibTeX

    HTML
    106

    Total
    115

    Share

    Search Panel

    Ayşe Başak Çakmen
    Google Scholar
    Pubmed
    JDMFS Journal

    Samir Abbas Ali Noma
    Google Scholar
    Pubmed
    JDMFS Journal

    Canbolat GÜRSES
    Google Scholar
    Pubmed
    JDMFS Journal

    Süleyman Köytepe
    Google Scholar
    Pubmed
    JDMFS Journal

    Burhan Ateş
    Google Scholar
    Pubmed
    JDMFS Journal

    İsmet Yılmaz
    Google Scholar
    Pubmed
    JDMFS Journal

    Downloads

    Article Details

    DOI: https://doi.org/10.37819/nanofab.8.1787

    Published: 2023-12-18

    Most Read This Month

    License

    Copyright (c) 2023 Ayşe Başak Çakmen, Samir Abbas Ali Noma, Canbolat GÜRSES, Süleyman Köytepe, Burhan Ateş, İsmet Yılmaz

    Creative Commons License

    This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

    Copyright © 2023 EurAsia Academic Publishing Group. All rights reserved